Publications

Shotaro Tanaka, Tokiko Saigo, Takekazu Kunieda, Yasuhiro Shiga, Fumio Nakamura (2026). A Versatile Technique for Measuring the Stiffness of Small Organisms Based on Microscopic Observation of Cantilever Strain. Zoological Science, 43(3),.

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Tokiko Saigo, Katsuya Satoh, Takekazu Kunieda (2025). Comparative Study of Gamma Radiation Tolerance between Desiccation-Sensitive and Desiccation-Tolerant Tardigrades. Zoological Science, 42(1), 107-115.

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Koyuki Kondo, Akihiro Tanaka, Takekazu Kunieda (2024). Single-Step Generation of Homozygous Knockout/Knock-in Individuals in an Extremotolerant Parthenogenetic Tardigrade Using DIPA-CRISPR. PLoS genetics, 20(6), e1011298.

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Kenta Sugiura, Yuki Yoshida, Kohei Hayashi, Kazuharu Arakawa, Takekazu Kunieda, Midori Matsumoto (2024). Sexual Dimorphism in the Tardigrade Paramacrobiotus Metropolitanus Transcriptome. Zoological Letters, 10(1), 11.

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Mohammad Al-Ansari, Taylor Fitzsimons, Wenbin Wei, Martin W. Goldberg, Takekazu Kunieda, Roy A. Quinlan (2024). The Major Inducible Small Heat Shock Protein HSP20-3 in the Tardigrade Ramazzottius Varieornatus Forms Filament-like Structures and Is an Active Chaperone. Cell Stress & Chaperones, 29(1), 51-65.

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Simon Galas, Emilie Le Goff, Chantal Cazevieille, Akihiro Tanaka, Pierre Cuq, Stephen Baghdiguian, Takekazu Kunieda, Nelly Godefroy, Myriam Richaud (2024). A Comparative Ultrastructure Study of the Tardigrade Ramazzottius Varieornatus in the Hydrated State, after Desiccation and during the Process of Rehydration. PloS One, 19(6), e0302552.

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Akihiro Tanaka, Takekazu Kunieda (2023). Considerations on the TardiVec-based Analyses of Tissue Specificity and Desiccation-Induced Supramolecular Structure of Target Proteins. Proceedings of the National Academy of Sciences of the United States of America, 120(48), e2312563120.

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Yasuhiro Matsumura, Taiko Kim To, Takekazu Kunieda, Hiroki Kohno, Tetsuji Kakutani, Takeo Kubo (2022). Mblk-1/E93, an Ecdysone Related-Transcription Factor, Targets Synaptic Plasticity-Related Genes in the Honey Bee Mushroom Bodies. Scientific Reports, 12(1), 21367.

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Hitomi Kumagai, Koyuki Kondo, Takekazu Kunieda (2022). Application of CRISPR/Cas9 System and the Preferred No-Indel End-Joining Repair in Tardigrades. Biochemical and Biophysical Research Communications, 623, 196-201.

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Akihiro Tanaka, Tomomi Nakano, Kento Watanabe, Kazutoshi Masuda, Gen Honda, Shuichi Kamata, Reitaro Yasui, Hiroko Kozuka-Hata, Chiho Watanabe, Takumi Chinen, Daiju Kitagawa, Satoshi Sawai, Masaaki Oyama, Miho Yanagisawa, Takekazu Kunieda (2022). Stress-Dependent Cell Stiffening by Tardigrade Tolerance Proteins That Reversibly Form a Filamentous Network and Gel. PLoS biology, 20(9), e3001780.

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Kenta Sugiura, Midori Matsumoto, Takekazu Kunieda (2022). Description of a Model Tardigrade Paramacrobiotus Metropolitanus Sp. Nov. (Eutardigrada) from Japan with a Summary of Its Life History, Reproduction and Genomics. Zootaxa, 5134(1), 92-112.

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Yuichiro Hara, Reira Shibahara, Koyuki Kondo, Wataru Abe, Takekazu Kunieda (2021). Parallel Evolution of Trehalose Production Machinery in Anhydrobiotic Animals via Recurrent Gene Loss and Horizontal Transfer. Open Biology, 11(7), 200413.

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Hitomi Kumagai, Takekazu Kunieda, Korefumi Nakamura, Yasuhiro Matsumura, Manami Namiki, Hiroki Kohno, Takeo Kubo (2020). Developmental Stage-Specific Distribution and Phosphorylation of Mblk-1, a Transcription Factor Involved in Ecdysteroid-Signaling in the Honey Bee Brain. Scientific Reports, 10(1), 8735.

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Satoshi Furukawa, Aiko Nagamatsu, Mitsuru Nenoi, Akira Fujimori, Shizuko Kakinuma, Takanori Katsube, Bing Wang, Chizuru Tsuruoka, Toshiyuki Shirai, Asako J. Nakamura, Asako Sakaue-Sawano, Atsushi Miyawaki, Hiroshi Harada, Minoru Kobayashi, Junya Kobayashi, Takekazu Kunieda, Tomoo Funayama, Michiyo Suzuki, Tatsuo Miyamoto, Jun Hidema, Yukari Yoshida, Akihisa Takahashi (2020). Space Radiation Biology for Living in Space. BioMed Research International, 2020, 4703286.

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Kenta Sugiura, Hiroki Minato, Atsushi C. Suzuki, Kazuharu Arakawa, Takekazu Kunieda, Midori Matsumoto (2019). Comparison of Sexual Reproductive Behaviors in Two Species of Macrobiotidae (Tardigrada: Eutardigrada). Zoological Science, 36(2), 120-127.

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Hiroshi Tsujioka, Takekazu Kunieda, Yuki Katou, Katsuhiko Shirahige, Taro Fukazawa, Takeo Kubo (2017). Interleukin-11 Induces and Maintains Progenitors of Different Cell Lineages during Xenopus Tadpole Tail Regeneration. Nature Communications, 8(1), 495.

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Yuki Yoshida, Georgios Koutsovoulos, Dominik R. Laetsch, Lewis Stevens, Sujai Kumar, Daiki D. Horikawa, Kyoko Ishino, Shiori Komine, Takekazu Kunieda, Masaru Tomita, Mark Blaxter, Kazuharu Arakawa (2017). Comparative Genomics of the Tardigrades Hypsibius Dujardini and Ramazzottius Varieornatus. PLoS biology, 15(7), e2002266.

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Takuma Hashimoto, Takekazu Kunieda (2017). DNA Protection Protein, a Novel Mechanism of Radiation Tolerance: Lessons from Tardigrades. Life, 7(2), 26.

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Seika Takayanagi-Kiya, Taketoshi Kiya, Takekazu Kunieda, Takeo Kubo (2017). Mblk-1 Transcription Factor Family: Its Roles in Various Animals and Regulation by NOL4 Splice Variants in Mammals. International Journal of Molecular Sciences, 18(2), 246.

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Atsuhiro Yamane, Hiroki Kohno, Tsubomi Ikeda, Kumi Kaneko, Atsushi Ugajin, Toshiyuki Fujita, Takekazu Kunieda, Takeo Kubo (2017). Gene Expression and Immunohistochemical Analyses of mKast Suggest Its Late Pupal and Adult-Specific Functions in the Honeybee Brain. PloS One, 12(5), e0176809.

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Yuko Hatta-Kobayashi, Mie Toyama-Shirai, Takehiro Yamanaka, Mayuko Takamori, Yoko Wakabayashi, Yuko Naora, Takekazu Kunieda, Taro Fukazawa, Takeo Kubo (2016). Acute Phase Response in Amputated Tail Stumps and Neural Tissue-Preferential Expression in Tail Bud Embryos of the Xenopus Neuronal Pentraxin I Gene. Development, Growth & Differentiation, 58(9), 688-701.

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Takuma Hashimoto, Daiki D. Horikawa, Yuki Saito, Hirokazu Kuwahara, Hiroko Kozuka-Hata, Tadasu Shin-I, Yohei Minakuchi, Kazuko Ohishi, Ayuko Motoyama, Tomoyuki Aizu, Atsushi Enomoto, Koyuki Kondo, Sae Tanaka, Yuichiro Hara, Shigeyuki Koshikawa, Hiroshi Sagara, Toru Miura, Shin-Ichi Yokobori, Kiyoshi Miyagawa, Yutaka Suzuki, Takeo Kubo, Masaaki Oyama, Yuji Kohara, Asao Fujiyama, Kazuharu Arakawa, Toshiaki Katayama, Atsushi Toyoda, Takekazu Kunieda (2016). Extremotolerant Tardigrade Genome and Improved Radiotolerance of Human Cultured Cells by Tardigrade-Unique Protein. Nature Communications, 7, 12808.

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Hiroshi Tsujioka, Takekazu Kunieda, Yuki Katou, Katsuhiko Shirahige, Takeo Kubo (2015). Unique Gene Expression Profile of the Proliferating Xenopus Tadpole Tail Blastema Cells Deciphered by RNA-sequencing Analysis. PloS One, 10(3), e0111655.

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Koyuki Kondo, Takeo Kubo, Takekazu Kunieda (2015). Suggested Involvement of PP1/PP2A Activity and De Novo Gene Expression in Anhydrobiotic Survival in a Tardigrade, Hypsibius Dujardini, by Chemical Genetic Approach. PloS One, 10(12), e0144803.

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Sae Tanaka, Junko Tanaka, Yoshihiro Miwa, Daiki D. Horikawa, Toshiaki Katayama, Kazuharu Arakawa, Atsushi Toyoda, Takeo Kubo, Takekazu Kunieda (2015). Novel Mitochondria-Targeted Heat-Soluble Proteins Identified in the Anhydrobiotic Tardigrade Improve Osmotic Tolerance of Human Cells. PloS One, 10(2), e0118272.

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Seika Takayanagi-Kiya, Kayo Misawa-Hojo, Taketoshi Kiya, Takekazu Kunieda, Takeo Kubo (2014). Splicing Variants of NOL4 Differentially Regulate the Transcription Activity of Mlr1 and Mlr2 in Cultured Cells. Zoological Science, 31(11), 735-740.

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Atsushi Ugajin, Takekazu Kunieda, Takeo Kubo (2013). Identification and Characterization of an Egr Ortholog as a Neural Immediate Early Gene in the European Honeybee (Apis Mellifera L.). FEBS letters, 587(19), 3224-3230.

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Yuko Naora, Yuko Hishida, Taro Fukazawa, Takekazu Kunieda, Takeo Kubo (2013). Expression Analysis of XPhyH-like during Development and Tail Regeneration in Xenopus Tadpoles: Possible Role of XPhyH-like Expressing Immune Cells in Impaired Tail Regenerative Ability. Biochemical and Biophysical Research Communications, 431(2), 152-157.

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Toshiyuki Fujita, Hiroko Kozuka-Hata, Hiroko Ao-Kondo, Takekazu Kunieda, Masaaki Oyama, Takeo Kubo (2013). Proteomic Analysis of the Royal Jelly and Characterization of the Functions of Its Derivation Glands in the Honeybee. Journal of Proteome Research, 12(1), 404-411.

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Kumi Kaneko, Tsubomi Ikeda, Mirai Nagai, Sayaka Hori, Chie Umatani, Hiroto Tadano, Atsushi Ugajin, Takayoshi Nakaoka, Rajib Kumar Paul, Tomoko Fujiyuki, Kenichi Shirai, Takekazu Kunieda, Hideaki Takeuchi, Takeo Kubo (2013). Novel Middle-Type Kenyon Cells in the Honeybee Brain Revealed by Area-Preferential Gene Expression Analysis. PloS One, 8(8), e71732.

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Daiki D. Horikawa, John Cumbers, Iori Sakakibara, Dana Rogoff, Stefan Leuko, Raechel Harnoto, Kazuharu Arakawa, Toshiaki Katayama, Takekazu Kunieda, Atsushi Toyoda, Asao Fujiyama, Lynn J. Rothschild (2013). Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius Varieornatus and Hypsibius Dujardini after Exposure to UVC Radiation. PloS One, 8(6), e64793.

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Taketoshi Kiya, Atsushi Ugajin, Takekazu Kunieda, Takeo Kubo (2012). Identification of Kakusei, a Nuclear Non-Coding RNA, as an Immediate Early Gene from the Honeybee, and Its Application for Neuroethological Study. International Journal of Molecular Sciences, 13(12), 15496-15509.

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Daiki D. Horikawa, Ayami Yamaguchi, Tetsuya Sakashita, Daisuke Tanaka, Nobuyuki Hamada, Fumiko Yukuhiro, Hirokazu Kuwahara, Takekazu Kunieda, Masahiko Watanabe, Yuichi Nakahara, Seiichi Wada, Tomoo Funayama, Chihiro Katagiri, Seigo Higashi, Shin-Ichi Yokobori, Mikinori Kuwabara, Lynn J. Rothschild, Takashi Okuda, Hirofumi Hashimoto, Yasuhiko Kobayashi (2012). Tolerance of Anhydrobiotic Eggs of the Tardigrade Ramazzottius Varieornatus to Extreme Environments. Astrobiology, 12(4), 283-289.

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Ayami Yamaguchi, Sae Tanaka, Shiho Yamaguchi, Hirokazu Kuwahara, Chizuko Takamura, Shinobu Imajoh-Ohmi, Daiki D. Horikawa, Atsushi Toyoda, Toshiaki Katayama, Kazuharu Arakawa, Asao Fujiyama, Takeo Kubo, Takekazu Kunieda (2012). Two Novel Heat-Soluble Protein Families Abundantly Expressed in an Anhydrobiotic Tardigrade. PloS One, 7(8), e44209.

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Atsushi Ugajin, Taketoshi Kiya, Takekazu Kunieda, Masato Ono, Tadaharu Yoshida, Takeo Kubo (2012). Detection of Neural Activity in the Brains of Japanese Honeybee Workers during the Formation of a Hot Defensive Bee Ball. PloS One, 7(3), e32902.

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Hirokazu Kuwahara, Yoshihiro Takaki, Shigeru Shimamura, Takao Yoshida, Taro Maeda, Takekazu Kunieda, Tadashi Maruyama (2011). Loss of Genes for DNA Recombination and Repair in the Reductive Genome Evolution of Thioautotrophic Symbionts of Calyptogena Clams. BMC evolutionary biology, 11, 285.

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Taro Fukazawa, Yuko Naora, Takekazu Kunieda, Takeo Kubo (2009). Suppression of the Immune Response Potentiates Tadpole Tail Regeneration during the Refractory Period. Development, 136(14), 2323-2327.

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Taketoshi Kiya, Takekazu Kunieda, Takeo Kubo (2008). Inducible- and Constitutive-Type Transcript Variants of Kakusei , a Novel Non-Coding Immediate Early Gene, in the Honeybee Brain. Insect Molecular Biology, 17(5), 531-536.

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Daiki D. Horikawa, Takekazu Kunieda, Wataru Abe, Masahiko Watanabe, Yuichi Nakahara, Fumiko Yukuhiro, Tetsuya Sakashita, Nobuyuki Hamada, Seiichi Wada, Tomoo Funayama, Chihiro Katagiri, Yasuhiko Kobayashi, Seigo Higashi, Takashi Okuda (2008). Establishment of a Rearing System of the Extremotolerant Tardigrade Ramazzottius Varieornatus: A New Model Animal for Astrobiology. Astrobiology, 8(3), 549-556.

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Ryoko Iijima, Takekazu Kunieda, Shinji Yamaguchi, Hiroko Kamigaki, Ikuko Fujii-Taira, Kazuhisa Sekimizu, Takeo Kubo, Shunji Natori, Koichi J. Homma (2008). The Extracellular Adenosine Deaminase Growth Factor, ADGF/CECR1, Plays a Role in Xenopus Embryogenesis via the Adenosine/P1 Receptor. The Journal of Biological Chemistry, 283(4), 2255-2264.

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Taketoshi Kiya, Takekazu Kunieda, Takeo Kubo (2007). Increased Neural Activity of a Mushroom Body Neuron Subtype in the Brains of Forager Honeybees. PloS One, 2(4), e371.

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Tomoko Ishino, Takekazu Kunieda, Shunji Natori, Kazuhisa Sekimizu, Takeo Kubo (2007). Identification of Novel Members of the Xenopus Ca2+ -Dependent Lectin Family and Analysis of Their Gene Expression during Tail Regeneration and Development. Journal of Biochemistry, 141(4), 479-488.

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Honeybee Genome Sequencing Consortium (2006). Insights into Social Insects from the Genome of the Honeybee Apis Mellifera. Nature, 443(7114), 931-949.

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Hideaki Takeuchi, Takekazu Kunieda, Miyuki Tokuhiro-Sawata, Jung-Min Park, Tomoko Fujiyuki, Takeo Kubo (2004). [Molecular ethology using the honeybee as a model animal]. Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme, 49(16), 2542-2548.

Takekazu Kunieda, Takeo Kubo (2004). In Vivo Gene Transfer into the Adult Honeybee Brain by Using Electroporation. Biochemical and Biophysical Research Communications, 318(1), 25-31.

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Jung-Min Park, Takekazu Kunieda, Takeo Kubo (2003). The Activity of Mblk-1, a Mushroom Body-Selective Transcription Factor from the Honeybee, Is Modulated by the Ras/MAPK Pathway. The Journal of Biological Chemistry, 278(20), 18689-18694.

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Tomoko Ishino, Mie Shirai, Takekazu Kunieda, Kazuhisa Sekimizu, Shunji Natori, Takeo Kubo (2003). Identification of Genes Induced in Regenerating Xenopus Tadpole Tails by Using the Differential Display Method. Developmental Dynamics: An Official Publication of the American Association of Anatomists, 226(2), 317-325.

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Takekazu Kunieda, Jung-Min Park, Hideaki Takeuchi, Takeo Kubo (2003). Identification and Characterization of Mlr1,2: Two Mouse Homologues of Mblk-1, a Transcription Factor from the Honeybee Brain(1). FEBS letters, 535(1-3), 61-65.

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Jung-Min Park, Takekazu Kunieda, Hideaki Takeuchi, Takeo Kubo (2002). DNA-binding Properties of Mblk-1, a Putative Transcription Factor from the Honeybee. Biochemical and Biophysical Research Communications, 291(1), 23-28.

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Hideaki Takeuchi, Eriko Kage, Miyuki Sawata, Azusa Kamikouchi, Kazuaki Ohashi, Maya Ohara, Tomoko Fujiyuki, Takekazu Kunieda, Kazuhisa Sekimizu, Shunji Natori, Takeo Kubo (2001). Identification of a Novel Gene, Mblk-1, That Encodes a Putative Transcription Factor Expressed Preferentially in the Large-Type Kenyon Cells of the Honeybee Brain. Insect Molecular Biology, 10(5), 487-494.

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Takekazu Kunieda, Shoichiro Kurata, Shunji Natori (1997). Regeneration of Sarcophaga Imaginal Discs in Vitro: Implication of 20-Hydroxyecdysone. Developmental Biology, 183(1), 86-94.

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